Previous Workshops
2016
Schedule
| Dates | 8:00 – 9:00AM | AM | PM | 4:30PM | |
| Day 1 22.08.2016 Monday |
Inauguration Lecture 1- Overview of molecular diagnosis-a clinician perspective -Dr. Vikram Mathews |
Introduction to the molecular lab DNA extraction (blood) DNA extraction from FFPE |
DNA extraction- CVS tissue | Group Discussion - DNA Extraction |
|
| Day 2 23.08.2016 Tuesday |
8:00 am: Establishing a molecular lab- Dr. Eunice Sindhuvi 8:30 am: Validation of molecular tests- Dr. Rekha Pai |
DNA quantity/quality check RNA extraction RNA quality and quantity check |
DNA quality check- Gel electrophoresis PCR and gel electrophoresis cDNA conversion |
Group Discussion - RNA Extraction/cDNA synthesis |
|
| Day 3 24.08.2016 Wednesday |
Lecture 3- Role of QC & QA in molecular lab – Dr. Eunice Sindhuvi |
House Keeping gene PCR Jak 2 PCR |
Gel electrophoresis Introduction to DNA sequencing VNTR Analysis PCR |
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| Day 4 25.08.2016 Thursday |
Lecture 4- Principles and Applications of Sequencing |
Sanger’s Sequencing Sequence reading/Trouble shooting Real Time PCR setup |
Analysis of Real time PCR Absolute quantitation/Relative Quantitation Sequencing printouts |
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| Day 5 26.08.2016 Friday |
Lecture 5- Methods in molecular diagnostics and latest advances – Dr.R.V.Shaji |
VNTR Analysis Application of F-PCR and capillary electrophoresis |
Bisulphite conversion and MGMT PCR |
Group Discussion – Sequencing / Real time PCR |
|
| Day 6 27.08.2016 Saturday |
Lecture 6- Principles and applications of NGS NGS Demonstration -Ilumina |
Next Generation Sequencing (Demo) | |||
| Day 7 29.08.2016 Monday |
Lecture 7 Digital Droplet PCR- Principle and Applications, Demo (Bio-Rad) |
Droplet Digital PCR (Demo) | Applications of a mass array system | Mol diagnosis of haemophilia | |
| Day 8 30.08.2016 Tuesday |
Lecture 8 Molecular Diagnosis of Leukemias- Dr.B.Poonkuzhali |
Molecular diagnosis of Leukemias | Approach to molecular diagnosis of hematological malignancies RT-PCR/Gel electrophoresis Group |
Discussion – Mol Diagnosis of Leukemias | |
| Day 9 31.08.2016 Wednesday |
Lecture 9 Molecular Diagnosis of Thalassemia – Dr.Anita Nadkarni, NIIH, Mumbai |
Molecular diagnosis of Thalassemia | Molecular diagnosis of Thalassemia -Beta thal, Alpha thal | Discussion | |
| Day 10 01.09.2016 Thursday |
Lecture 10- Solid tumours Molecular analysis of Solid tumors- Dr. Rekha Pai |
Molecular diagnosis of solid tumours- Set up TCRG To set up MSI testing |
Molecular diagnosis of solid tumours |
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| Day 11 02.09.2016 Friday |
Lecture 11- Molecular Diagnosis of solid tumors- clinical perspective - Dr. Raju Titus Chacko |
Final discussion and case presentations | |||
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